Membrane Lipids: Methods and Protocols (Methods in Molecular Biology Book 2402)
Membrane Lipids: Methods and Protocols (Methods in Molecular Biology, Volume 2402) is a definitive laboratory manual that compiles advanced and reliable protocols for the isolation, analysis, and functional study of membrane lipids. This essential volume is part of the renowned Methods in Molecular Biology series and is now available at Books Hub PK for researchers, academic professionals, and students in biochemistry, molecular biology, and lipidomics.
Membrane lipids are not just structural elements—they are active players in cell signaling, trafficking, and metabolic regulation. Understanding their complex roles requires robust, reproducible techniques. This book offers a comprehensive toolkit covering both traditional and next-generation methods for exploring lipid composition and dynamics.
Curated by leading experts in the field, this book includes:
Extraction and purification of lipids from diverse biological samples
High-performance thin-layer chromatography (HPTLC)
Gas chromatography-mass spectrometry (GC-MS)
Liquid chromatography-tandem mass spectrometry (LC-MS/MS)
Lipidomics data analysis and interpretation
Imaging techniques for visualizing membrane lipid distribution
Functional assays to study lipid-protein interactions
Each chapter provides a step-by-step protocol, complete with materials lists, critical parameters, data interpretation tips, and troubleshooting suggestions, making it an ideal resource for both novice and experienced laboratory researchers.
