Embryonic Stem Cell Protocols (Methods in Molecular Biology 2520) 4th Edition
Embryonic Stem Cell Protocols (Methods in Molecular Biology, Volume 2520) – 4th Edition is an essential laboratory manual offering the most up-to-date, standardized methodologies in the dynamic field of embryonic stem cell (ESC) research. This trusted volume, part of the prestigious Methods in Molecular Biology series, is now available for purchase at Books Hub PK, your reliable source for authentic academic and research books across Pakistan.
Curated by global experts, this newly revised edition delivers robust and reproducible protocols that reflect the latest advancements in ESC isolation, maintenance, differentiation, and genetic modification. Whether you’re working with mouse, human, or non-human primate ESCs, this book serves as a complete technical reference, designed to support researchers, educators, and postgraduate students engaged in developmental biology, regenerative medicine, and cellular therapy.
🔬 What’s Inside:
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Derivation and culture of mouse and human embryonic stem cells
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Genome editing in ESCs using CRISPR/Cas9
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In vitro differentiation into mesodermal, endodermal, and ectodermal lineages
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Transcriptomics and proteomics analysis
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Epigenetic regulation and reprogramming
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Teratoma assays and chimera formation protocols
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Quality control and troubleshooting guidance
Each protocol is presented with a clear step-by-step format, accompanied by reagent lists, detailed methodologies, expert tips, and troubleshooting notes to ensure maximum reproducibility and success in the lab.
