DNA-Encoded Chemical Libraries: Methods and Protocols (Methods in Molecular Biology 2541)

Description

DNA-Encoded Chemical Libraries: Methods and Protocols (Methods in Molecular Biology 2541)

Unlock the future of drug discovery with DNA‑Encoded Chemical Libraries: Methods and Protocols, Volume 2541 from the renowned Methods in Molecular Biology series—now available at Books Hub PK. This authoritative manual equips scientists, pharmaceutical researchers, and biotech professionals with the detailed laboratory protocols required to design, synthesize, screen, and optimize ultra-large, DNA-tagged small-molecule libraries using state-of-the-art DEL technology.

Emerging as a revolutionary tool in modern medicinal chemistry, DNA‑encoded chemical libraries (DELs) allow the creation and simultaneous screening of millions to billions of compounds in a single test tube, encoded with unique DNA barcodes. Through affinity-based selection and PCR/barcode sequencing, DELs facilitate rapid identification of high-affinity ligands—even against difficult targets.

🧪 Key Features:

• Library Synthesis: Advanced methods for split‑and‑pool assembly, on-DNA click chemistry, Suzuki coupling, reductive amination, and more.

• Encoding Mechanics: Protocols for DNA conjugation and barcode design to ensure precise compound tracking.

• Screening Strategies: Step-wise affinity-based DEL selections against protein targets, including qPCR-mediated analysis and high-throughput sequencing for hit identification.

• Computational Tools: Practical guidelines for selecting hit compounds for resynthesis and downstream validation.

• Reproducibility & Troubleshooting: Expert tips, reagents, and implementation advice for robust results.

🎯 Ideal For:

• Medicinal chemists and biochemists exploring novel ligand discovery.

• Drug development teams engaged in hit identification and optimization.

• Molecular biologists and protein scientists using DELs to target challenging therapeutic proteins.

• Graduate students and postdocs in drug discovery, pharmacology, and chemical biology.

✅ Why Buy from Books Hub PK:

• Genuine international edition with full technical accuracy.

• Competitive pricing tailored to Pakistani researchers and educational institutions.

• Fast nationwide delivery with Cash-on-Delivery (COD) option.

• Trusted by academic and research communities for original scientific resources in Pakistan.

🌟 Why Invest in This Book?

• DEL technology is transforming ligand discovery by enabling screening of libraries at trillions‑scale, using just nanogram quantities of target protein—dramatically reducing time and cost compared to traditional screening methods.

• DEL platforms allow discovery of ligands for targets previously considered “undruggable”, including protein–protein interfaces and signaling proteins.

• Packed with laboratory-tested, step-by-step protocols, this volume bridges the gap between theory and practice in next-gen small molecule screening.

Equip your lab with the essential manual for DNA‑Encoded Chemical Libraries—a powerful toolkit to drive innovation in drug discovery, molecular screening, and chemical biology.

🛒 Order your copy of DNA‑Encoded Chemical Libraries: Methods and Protocols (Vol. 2541) today from Books Hub PK and be among the pioneers leveraging DEL technology in Pakistan’s research landscape!