HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols (Methods in Molecular Biology Book 2589) 2nd Edition
HDAC/HAT Function Assessment and Inhibitor Development: Methods and Protocols (Methods in Molecular Biology Book 2589, 2nd Edition) is a comprehensive laboratory guide designed for researchers, biochemists, pharmacologists, and molecular biologists working in the field of epigenetics, chromatin biology, and drug discovery. This updated edition offers step-by-step protocols for analyzing histone deacetylase (HDAC) and histone acetyltransferase (HAT) activities, along with cutting-edge methods for screening and developing small-molecule inhibitors.
Part of the prestigious Methods in Molecular Biology series, this volume provides detailed experimental procedures, reagent lists, troubleshooting tips, and reproducible methodologies—ensuring reliable results for both basic and translational research. It covers in vitro biochemical assays, cell-based functional studies, structural analysis, and computational approaches for inhibitor development, making it an indispensable tool for drug discovery programs targeting epigenetic enzymes.
Key Features:
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Updated protocols for HDAC and HAT enzyme activity assessment.
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High-throughput screening techniques for inhibitor identification.
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Structural and computational methods for rational drug design.
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Cellular and in vivo models for functional validation.
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Expert tips for maximizing reproducibility and minimizing errors.
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Part of the Methods in Molecular Biology trusted series, ensuring quality and reliability.