Estrogen Receptors: Methods and Protocols (Methods in Molecular Biology Book 2418) 2nd Edition
Advance your research on hormone signaling and receptor biology with the 2nd Edition of “Estrogen Receptors: Methods and Protocols” – part of the esteemed Methods in Molecular Biology series (Book 2418). Available now at Books Hub PK, this updated volume serves as a comprehensive laboratory reference for scientists, researchers, and students working in molecular endocrinology, oncology, reproductive biology, and pharmacological research.
Estrogen receptors (ERs), particularly ERα and ERβ, play crucial roles in various physiological processes including reproduction, metabolism, cardiovascular health, and cancer progression. This book presents a detailed collection of cutting-edge experimental techniques used to study the structure, expression, function, and therapeutic targeting of ERs in both in vitro and in vivo systems.
Key Features of the 2nd Edition:
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Step-by-step protocols for detecting, quantifying, and analyzing estrogen receptor activity
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Techniques include RT-qPCR, chromatin immunoprecipitation (ChIP), ligand-binding assays, gene reporter analysis, and RNA sequencing
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Methods for tissue-specific localization, receptor knockdown, and functional studies in cell lines and animal models
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Covers both genomic and non-genomic estrogen signaling pathways
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Detailed guidance on troubleshooting, reagent preparation, and data interpretation
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Contributions from leading experts in estrogen signaling and receptor biology
Each protocol is presented in the time-tested format of the Methods in Molecular Biology series, including background information, materials, step-by-step procedures, and expert tips—ideal for both beginners and experienced researchers.