Fluorescent Proteins: Methods and Protocols (Methods in Molecular Biology)
Unlock the power of fluorescent proteins in biomedical research and molecular biology with “Fluorescent Proteins: Methods and Protocols”, part of the highly regarded Methods in Molecular Biology series (Volume 2540). This comprehensive laboratory manual is an essential resource for molecular biologists, biochemists, cell biologists, and life science researchers working in protein visualization, cellular imaging, and live-cell tracking.
Fluorescent proteins have transformed modern biology by enabling real-time visualization of cellular processes, protein-protein interactions, and gene expression. This volume compiles cutting-edge protocols, practical methodologies, and advanced imaging techniques contributed by leading experts in the field.
Key Features:
-
Step-by-step protocols for expression, purification, and imaging of fluorescent proteins
-
Detailed sections on GFP, mCherry, CFP, YFP, and other fluorescent protein variants
-
Protocols for live-cell imaging, FRET analysis, photobleaching, and super-resolution microscopy
-
Guidelines for construct design, gene fusion strategies, and reporter assays
-
Tips for troubleshooting, optimizing fluorescence, and avoiding photobleaching
-
Applications in cancer research, neuroscience, developmental biology, and microbiology
-
Includes reproducible techniques, materials lists, and expert notes for successful lab execution
Whether you’re working with bacterial systems, mammalian cells, or whole organisms, this book provides the technical know-how to design and implement experiments using fluorescent proteins confidently.
