Mammary Stem Cells: Methods and Protocols (Methods in Molecular Biology 2471) 2nd Edition
Advance your research in breast biology, regenerative medicine, and cancer with the 2nd Edition of “Mammary Stem Cells: Methods and Protocols” (Methods in Molecular Biology, Volume 2471)—now available at Books Hub PK. This updated and expanded edition offers a complete, hands-on guide to the isolation, culture, characterization, and manipulation of mammary stem cells (MaSCs), placing powerful and proven methods in the hands of researchers.
As mammary stem cells play a critical role in breast development, tissue regeneration, and the pathogenesis of breast cancer, this volume provides cutting-edge techniques that are essential for basic science and translational medicine. Whether you’re investigating cell lineage, tumor heterogeneity, or therapeutic resistance, these protocols support high-quality, reproducible research.
Inside This Edition:
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Protocols for mammary gland dissection, cell sorting (FACS), and 3D culture models
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In vivo methods for transplantation, lineage tracing, and mouse mammary gland assays
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CRISPR/Cas9 gene editing and lentiviral transduction techniques
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Methods to study cell signaling, differentiation pathways, and stemness markers
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Imaging and flow cytometry-based analysis of stem/progenitor cell populations
Each chapter is written by domain experts and follows the time-tested Methods in Molecular Biology format, featuring detailed instructions, required materials, tips for success, and troubleshooting guidance.
