Optical Tweezers: Methods and Protocols (Methods in Molecular Biology Book 2478) 2nd Edition
“Optical Tweezers: Methods and Protocols (2nd Edition)” from the prestigious Methods in Molecular Biology series (Book 2478) is a cutting-edge laboratory guide offering detailed, hands-on techniques for scientists working with laser-based single-molecule manipulation tools. Edited by leading researchers in the field, this comprehensive volume combines theoretical background with practical laboratory protocols, making it an indispensable resource for physicists, biologists, biophysicists, and engineers.
Optical tweezers are now widely used in molecular and cellular biology, biophysics, and nanotechnology to explore interactions at the single-molecule level. This fully updated second edition includes new experimental protocols, technological advancements, and improvements in instrumentation, covering the full range of applications from force measurements to molecular motor analysis.
Inside This Book:
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Detailed protocols for trapping and manipulating microscopic particles
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Methods for calibrating optical tweezers, including stiffness and force sensitivity
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Applications in DNA-protein interactions, protein unfolding, molecular motors, and cell mechanics
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Step-by-step workflows, materials lists, and expert tips for reproducibility
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Troubleshooting notes and adaptable techniques for various experimental setups
Every chapter is written by an expert with first-hand experience, and includes an introduction to the principles, followed by a clear methodology, and relevant references for deeper exploration.
