Mitosis: Methods and Protocols (Methods in Molecular Biology 2415)
Advance your understanding of cell division with “Mitosis: Methods and Protocols (Methods in Molecular Biology, Book 2415)”, now available at Books Hub PK. This authoritative volume in the highly respected Methods in Molecular Biology series presents a comprehensive suite of laboratory protocols for investigating the complex and tightly regulated process of mitosis, the foundation of cellular reproduction and genetic stability.
Mitosis is a critical event in the cell cycle, ensuring the equal segregation of chromosomes into daughter cells. Errors in mitotic processes can lead to genomic instability, which underlies various diseases, including cancer. This book brings together cutting-edge methods and best practices contributed by leading experts to study mitotic spindle assembly, chromosome alignment, checkpoint regulation, and cytokinesis.
This volume covers a range of molecular, imaging, genetic, and biochemical approaches, including:
Live-cell imaging of mitosis
CRISPR/Cas9-based gene editing
Kinase activity assays
Proteomics and phosphoproteomics in mitotic regulation
Mitotic checkpoint function analysis
Each chapter includes step-by-step experimental procedures, lists of required reagents and equipment, and troubleshooting tips, making it suitable for both novice researchers and experienced molecular biologists. These protocols are designed for high reproducibility, saving researchers valuable time in optimizing experiments.
Key Features:
Practical techniques for studying spindle dynamics, chromosome behavior, and mitotic signaling
Applications in cancer biology, cell cycle research, and drug discovery
Covers both mammalian and model organism systems
High-resolution microscopy and live-cell tracking protocols
Full-color illustrations, data analysis strategies, and expert commentary
This book is a must-have for cell biologists, molecular biologists, cancer researchers, and biomedical scientists focused on understanding mitotic regulation, genetic fidelity, and therapeutic targeting. It is especially valuable for PhD students, postdoctoral researchers, and lab professionals working in cell cycle biology and molecular oncology.
