Insoluble Proteins: Methods and Protocols (Methods in Molecular Biology 2406) 2nd Edition
Explore the complexities of protein biochemistry with “Insoluble Proteins: Methods and Protocols – 2nd Edition (Methods in Molecular Biology, Volume 2406)”, now available at Books Hub PK. This extensively updated volume provides state-of-the-art laboratory protocols for the study, solubilization, and characterization of insoluble proteins, a major challenge in molecular biology and biopharmaceutical research.
Insoluble proteins, including aggregates, inclusion bodies, and membrane proteins, play essential roles in various biological systems and diseases—yet are notoriously difficult to handle. This book equips researchers with practical and reliable methods to analyze proteins that do not readily dissolve in aqueous solutions, making it a vital resource for tackling structural and functional questions in biochemistry, proteomics, and structural biology.
Edited and authored by field-leading scientists, the protocols cover:
Solubilization and refolding of inclusion bodies
Extraction and purification of membrane-associated proteins
Advanced detergent- and non-detergent-based methods
Analytical tools like mass spectrometry, circular dichroism, and NMR
Expression systems including E. coli, yeast, insect, and mammalian cells
Each chapter includes step-by-step procedures, reagent details, expert tips, and data interpretation guidelines, ensuring high reproducibility and usability in academic, industrial, and clinical research labs.
Key Features:
Covers modern tools for solubilizing and analyzing insoluble and aggregation-prone proteins
Protocols adaptable to various protein types and research goals
Applications in drug development, vaccine production, and structural proteomics
Ideal for both basic and translational research
Includes troubleshooting insights and optimization strategies
This volume is indispensable for molecular biologists, protein chemists, structural biologists, and biotechnology researchers. Whether you’re studying misfolded proteins in neurodegenerative diseases, working with recombinant expression systems, or optimizing biopharmaceutical production, this book offers expert-level guidance.
