Protoplast Technology: Methods and Protocols (Methods in Molecular Biology 2464)

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Protoplast Technology: Methods and Protocols (Methods in Molecular Biology 2464)

Explore the powerful world of plant biotechnology with “Protoplast Technology: Methods and Protocols (Methods in Molecular Biology, Volume 2464)”, now available at Books Hub PK. This advanced laboratory manual delivers a comprehensive suite of proven protocols for plant protoplast isolation, transformation, fusion, and analysis, empowering researchers across plant science, molecular biology, and genetic engineering.

Protoplasts—plant cells with their cell walls enzymatically removed—are key to advancing plant molecular genetics, genome editing, and cellular reprogramming. This volume offers cutting-edge techniques that make protoplast-based experimentation efficient, reproducible, and scalable for both model plants and crop species.

Edited by leading experts in plant cell biology, this book includes:

  • Protocols for isolating viable protoplasts from various plant tissues

  • Methods for PEG-mediated transformation and electroporation

  • Techniques for CRISPR/Cas9-based genome editing in protoplasts

  • Protoplast fusion, regeneration, and viability assays

  • Fluorescence microscopy, flow cytometry, and live-cell imaging applications

Every chapter features step-by-step instructions, reagent lists, critical troubleshooting tips, and expected results—making it ideal for both novice and experienced researchers working in academic, biotech, or agricultural labs.

Key Features:

  • Covers both model species (e.g., Arabidopsis) and economically important crops

  • Includes protocols for gene expression studies, transient assays, and subcellular localization

  • Offers reliable methods for high-throughput protoplast screening

  • Perfect for functional genomics, synthetic biology, and crop improvement projects

Whether you’re developing transgenic lines, optimizing gene editing workflows, or conducting cell signaling experiments, this volume equips you with the essential tools to succeed in protoplast-based plant research.

Description

Protoplast Technology: Methods and Protocols (Methods in Molecular Biology 2464)

Explore the powerful world of plant biotechnology with “Protoplast Technology: Methods and Protocols (Methods in Molecular Biology, Volume 2464)”, now available at Books Hub PK. This advanced laboratory manual delivers a comprehensive suite of proven protocols for plant protoplast isolation, transformation, fusion, and analysis, empowering researchers across plant science, molecular biology, and genetic engineering.

Protoplasts—plant cells with their cell walls enzymatically removed—are key to advancing plant molecular genetics, genome editing, and cellular reprogramming. This volume offers cutting-edge techniques that make protoplast-based experimentation efficient, reproducible, and scalable for both model plants and crop species.

Edited by leading experts in plant cell biology, this book includes:

  • Protocols for isolating viable protoplasts from various plant tissues

  • Methods for PEG-mediated transformation and electroporation

  • Techniques for CRISPR/Cas9-based genome editing in protoplasts

  • Protoplast fusion, regeneration, and viability assays

  • Fluorescence microscopy, flow cytometry, and live-cell imaging applications

Every chapter features step-by-step instructions, reagent lists, critical troubleshooting tips, and expected results—making it ideal for both novice and experienced researchers working in academic, biotech, or agricultural labs.

Key Features:

  • Covers both model species (e.g., Arabidopsis) and economically important crops

  • Includes protocols for gene expression studies, transient assays, and subcellular localization

  • Offers reliable methods for high-throughput protoplast screening

  • Perfect for functional genomics, synthetic biology, and crop improvement projects

Whether you’re developing transgenic lines, optimizing gene editing workflows, or conducting cell signaling experiments, this volume equips you with the essential tools to succeed in protoplast-based plant research.

Ideal For:

  • Plant molecular biologists

  • Genetic engineers and biotechnologists

  • Crop scientists and agronomists

  • Graduate students and academic researchers

  • Researchers in pharmaceutical and agri-tech sectors

Why Buy from Books Hub PK?
Books Hub PK is your reliable partner for authentic scientific literature, delivering original Springer editions across Pakistan at competitive prices.

Official Springer Edition – Volume 2464 in MMB Series
Essential Toolkit for Plant Molecular Biology & Genetic Engineering
Step-by-Step Protocols for Protoplast Isolation & Transformation
Fast Nationwide Delivery with Trusted Service

Order “Protoplast Technology: Methods and Protocols (Methods in Molecular Biology, 2464)” today from Books Hub PK and unlock the full potential of plant protoplast systems in your research.

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