Protoplast Technology: Methods and Protocols (Methods in Molecular Biology 2464)
Explore the powerful world of plant biotechnology with “Protoplast Technology: Methods and Protocols (Methods in Molecular Biology, Volume 2464)”, now available at Books Hub PK. This advanced laboratory manual delivers a comprehensive suite of proven protocols for plant protoplast isolation, transformation, fusion, and analysis, empowering researchers across plant science, molecular biology, and genetic engineering.
Protoplasts—plant cells with their cell walls enzymatically removed—are key to advancing plant molecular genetics, genome editing, and cellular reprogramming. This volume offers cutting-edge techniques that make protoplast-based experimentation efficient, reproducible, and scalable for both model plants and crop species.
Edited by leading experts in plant cell biology, this book includes:
Protocols for isolating viable protoplasts from various plant tissues
Methods for PEG-mediated transformation and electroporation
Techniques for CRISPR/Cas9-based genome editing in protoplasts
Protoplast fusion, regeneration, and viability assays
Fluorescence microscopy, flow cytometry, and live-cell imaging applications
Every chapter features step-by-step instructions, reagent lists, critical troubleshooting tips, and expected results—making it ideal for both novice and experienced researchers working in academic, biotech, or agricultural labs.
Key Features:
Covers both model species (e.g., Arabidopsis) and economically important crops
Includes protocols for gene expression studies, transient assays, and subcellular localization
Offers reliable methods for high-throughput protoplast screening
Perfect for functional genomics, synthetic biology, and crop improvement projects
Whether you’re developing transgenic lines, optimizing gene editing workflows, or conducting cell signaling experiments, this volume equips you with the essential tools to succeed in protoplast-based plant research.