Essential Genes and Genomes: Methods and Protocols (Methods in Molecular Biology Book 2377)
Dive into the heart of functional genomics with “Essential Genes and Genomes: Methods and Protocols” (Methods in Molecular Biology, Volume 2377)—a cutting-edge laboratory manual that provides comprehensive methodologies for identifying and characterizing essential genes across a wide range of organisms. Available now at Books Hub PK, this volume is an invaluable resource for researchers in genomics, molecular biology, synthetic biology, and systems biology.
Understanding which genes are essential to life underpins nearly all areas of biological and biomedical research—from drug target discovery to metabolic engineering and minimal genome design. This book delivers a wide array of reproducible, peer-reviewed protocols designed to support both experimental and computational exploration of gene essentiality.
Key Features:
Covers transposon mutagenesis (Tn-seq), CRISPR-based gene editing, RNAi screening, and genome-scale knockout strategies
Includes protocols for bacterial, yeast, and mammalian model systems
Highlights computational methods for essential gene prediction using machine learning and comparative genomics
Offers insights into functional annotation, fitness profiling, and gene-network analysis
Step-by-step experimental methods with materials lists, detailed workflows, and troubleshooting tips
Emphasis on reproducibility, data validation, and integration with omics datasets
Each protocol follows the trusted Methods in Molecular Biology format: Introduction, Materials, Methods, and Notes—making it accessible for both early-career researchers and experienced scientists.
