Alternative Splicing: Methods and Protocols (Methods in Molecular Biology Book 2537)
Advance your research in gene regulation and RNA processing with the newly published Alternative Splicing: Methods and Protocols (Book 2537) from the renowned Methods in Molecular Biology series—now available at Books Hub PK. This essential laboratory guide delivers a comprehensive collection of cutting-edge protocols for studying one of the most crucial post-transcriptional processes: alternative splicing.
Edited and authored by international experts in molecular biology and RNA research, this 2024 edition provides a solid framework to analyze, manipulate, and interpret splicing events across various organisms. It is especially valuable in understanding how a single gene can generate multiple proteins, a key concept in developmental biology, neurobiology, cancer research, and personalized medicine.
Whether you’re conducting research in academia, biotech, or pharmaceutical labs, this volume offers reproducible techniques optimized for both wet-lab and computational settings, helping you stay at the forefront of RNA biology.
🔬 Key Features Include:
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High-throughput RNA sequencing (RNA-Seq) for splicing analysis
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Minigene splicing reporter assays
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In vitro splicing systems and spliceosome assembly analysis
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CRISPR/Cas9-based genome editing for splicing regulation
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Quantitative RT-PCR and bioinformatic tools
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Splice isoform detection, quantification, and functional analysis
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Troubleshooting tips, reagent lists, and step-by-step guidance
Each chapter is designed in the classic Methods in Molecular Biology format, ensuring clarity, precision, and replicability for researchers at all levels.
