Endocannabinoid Signaling: Methods and Protocols (Methods in Molecular Biology Book 2576) 2nd Edition
Endocannabinoid Signaling: Methods and Protocols – 2nd Edition is an advanced, practical guide for neuroscientists, pharmacologists, molecular biologists, and biomedical researchers working in the dynamic field of endocannabinoid research. As part of the world-renowned Methods in Molecular Biology series, this updated edition delivers state-of-the-art laboratory protocols and analytical methods for investigating the endocannabinoid system (ECS) in both physiological and pathological contexts.
The endocannabinoid system plays a crucial role in regulating neural signaling, immune function, pain perception, metabolism, and mood regulation. This book equips researchers with step-by-step experimental techniques for studying endocannabinoid biosynthesis, degradation, receptor interactions, and signaling pathways.
Key Features:
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Comprehensive coverage of ECS research – Including lipid extraction, enzyme assays, receptor binding studies, and signaling pathway analysis.
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Updated protocols for advanced technologies – Such as LC-MS/MS for endocannabinoid quantification, genetic manipulation, and optogenetic tools.
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In vivo and in vitro methodologies – Suitable for animal models, cell cultures, and human tissue samples.
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Applications in biomedical research – Covers roles of ECS in pain, neurodegeneration, inflammation, psychiatric disorders, and cancer.
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Expert contributors – Authored by leading scientists in neurobiology, pharmacology, and molecular signaling.
