Chromaffin Cells: Methods and Protocols (Methods in Molecular Biology 2565)
Chromaffin Cells: Methods and Protocols is a definitive laboratory guide for neuroscientists, endocrinologists, and cell biologists interested in the biology, physiology, and pathology of chromaffin cells—specialized neuroendocrine cells of the adrenal medulla responsible for catecholamine secretion. As part of the world-renowned Methods in Molecular Biology series, this volume delivers step-by-step experimental procedures that make cutting-edge research both reproducible and accessible.
Chromaffin cells are central to understanding neurotransmitter release, neuroendocrine signaling, and stress responses. This book equips researchers with modern techniques for isolating, culturing, and functionally analyzing these cells, while providing troubleshooting tips for optimal results in both basic research and clinical applications.
Key Features:
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Complete Methodology – Covers isolation from adrenal glands, primary culture techniques, and advanced imaging of exocytosis.
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Functional Assays – Protocols for studying catecholamine release, ion channel activity, and vesicle trafficking.
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Cutting-Edge Tools – Incorporates patch-clamp electrophysiology, live-cell imaging, and molecular manipulation techniques.
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Troubleshooting Sections – Expert advice to overcome common experimental hurdles.
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Relevance to Human Health – Methods adaptable to research on stress disorders, hypertension, and neurodegenerative diseases.
