Phase-Separated Biomolecular Condensates: Methods and Protocols (Methods in Molecular Biology)
Phase-Separated Biomolecular Condensates: Methods and Protocols is a cutting-edge laboratory manual in the Methods in Molecular Biology series, designed to equip researchers with the latest tools and techniques for studying membraneless organelles and biomolecular phase separation. This book bridges molecular biology, biophysics, and cell biology, offering an in-depth look at the mechanisms by which proteins and nucleic acids form dynamic, liquid-like compartments within cells.
Edited by leaders in the field, this volume presents step-by-step, reproducible protocols that are both beginner-friendly and sufficiently detailed for advanced research projects. It is a must-have reference for scientists studying condensate biology, cellular organization, and disease-related phase separation phenomena.
Key Features:
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Comprehensive Techniques – Covers in vitro reconstitution, fluorescence microscopy, FRAP, FCS, and biophysical characterization.
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Cell-Based Studies – Methods for visualizing and analyzing condensates in live cells.
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Protein & RNA Condensates – Protocols tailored for diverse biomolecular systems.
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Troubleshooting Sections – Expert insights to help you avoid common experimental pitfalls.
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Applications in Disease Research – Explore links to neurodegeneration, cancer, and viral infection.