R-Loops: Methods and Protocols (Methods in Molecular Biology Book 2528)

Explore the latest research tools and protocols in genome biology and transcriptional regulation with R-Loops: Methods and Protocols (Methods in Molecular Biology, Vol. 2528), now available for purchase at Books Hub PK. This authoritative laboratory manual is an essential addition to the bookshelf of molecular biologists, genomic scientists, and biomedical researchers exploring R-loop biology—a rapidly expanding field with critical implications in gene expression, genome instability, and disease pathology.

R-loops, the unique three-stranded nucleic acid structures formed during transcription, have become pivotal in understanding epigenetic regulation, DNA repair, cancer biology, and neurodegenerative disorders. This volume compiles state-of-the-art laboratory techniques for detecting, mapping, quantifying, and manipulating R-loops, enabling researchers to dive deep into their structural and functional dynamics.

🔍 Key Features:

• Comprehensive protocols for genome-wide and locus-specific R-loop mapping, including DRIP-seq and S9.6-based detection

• Guidelines for in vitro and in vivo analysis of R-loop dynamics

• Advanced biochemical, molecular, and imaging techniques for high-resolution structural characterization

• Tools to explore the impact of R-loops in genome stability, DNA damage response, and gene regulation

• Practical tips for optimizing experiments, troubleshooting errors, and ensuring reproducibility

• Contributions from internationally recognized experts and thought leaders in nucleic acid biology