T-Follicular Helper Cells: Methods and Protocols (Methods in Molecular Biology 2380)

T-Follicular Helper Cells: Methods and Protocols (Methods in Molecular Biology, Volume 2380) is an essential resource for immunologists and biomedical researchers exploring the pivotal role of T-follicular helper (Tfh) cells in adaptive immunity, autoimmunity, and vaccine response. Now available at Books Hub PK, this expertly curated laboratory manual provides detailed, peer-reviewed protocols tailored to cutting-edge immunological research.

Tfh cells play a central role in B-cell maturation and antibody production within germinal centers. This volume offers a comprehensive toolkit of experimental protocols for isolating, characterizing, and functionally analyzing Tfh cells across mouse and human models. Edited and written by leaders in the field, it provides precise guidance to study Tfh cell differentiation, gene expression, cytokine profiling, and functional assays related to immune regulation.

Each chapter presents a step-by-step method, including materials, reagents, expected results, and troubleshooting tips to ensure experimental success and reproducibility. Special focus is given to flow cytometry, RNA sequencing, gene editing, adoptive transfers, and in vivo functional studies—making it ideal for both basic and translational immunology research.

Key Features:

  • Focused coverage on Tfh cell biology and methodology

  • Techniques for cell sorting, phenotyping, and in vivo modeling

  • Protocols for analyzing cytokine production, gene expression, and helper function

  • Methods for both murine and human-derived Tfh cells

  • Includes troubleshooting sections and expert commentary

  • Fully indexed and illustrated for ease of reference

This book is an indispensable guide for researchers investigating autoimmune diseases, vaccine development, B-cell immunity, and lymphoid tissue architecture. Whether you're designing experiments for novel immunotherapies or exploring the molecular mechanisms of Tfh function, this volume provides a practical and reliable foundation for your lab work.