The Unfolded Protein Response: Methods and Protocols (Methods in Molecular Biology Book 2378)

Advance your molecular biology and cellular stress research with The Unfolded Protein Response: Methods and Protocols, edited by Roberto Pérez‑Torrado and published by Springer Nature in 2022. Part of the acclaimed Methods in Molecular Biology series, this authoritative handbook delivers detailed, reproducible laboratory methods for studying the unfolded protein response (UPR)—a critical cellular pathway in ER stress, disease, and homeostasis.

🔬 Key Features & Contents

  • Six Comprehensive Sections covering:

    • Measurement of unfolded protein levels

    • UPR signaling pathways (PERK, IRE1, ATF6)

    • Downstream responses including apoptosis, chaperone induction, and ER‑associated degradation

    • Mammalian and alternative model systems (yeast, plant protoplasts)

    • UPR in human disease contexts (neurodegenerative disorders, metabolic conditions)

  • Reproducible Protocol Format: Each chapter includes introduction, materials, step-by-step methods, troubleshooting tips, and expert notes for effective execution in your lab.

  • In Planta UPR Methods: Specific chapters detail protocols such as protein extraction for proteomics in Arabidopsis thaliana under ER stress conditions.

  • Fluorescence & Biosensor Assays: Detect protein unfolding using FRET‑based sensors and fluorescent reporter constructs in real time.

  • Cross-Model Coverage: From yeast (HAC1 mRNA splicing assays), mammalian cell culture, to plant systems—this volume supports research across biological kingdoms.