Vasculogenic Mimicry: Methods and Protocols (Methods in Molecular Biology 2514)

Delve into the cutting-edge field of tumor biology with "Vasculogenic Mimicry: Methods and Protocols", part of the esteemed Methods in Molecular Biology series (Vol. 2514). This volume is a crucial resource for cancer researchers, cellular biologists, and molecular medicine specialists exploring the alternative pathways of tumor vascularization beyond traditional angiogenesis.

Vasculogenic mimicry (VM) represents a tumor-driven vascular strategy, where aggressive cancer cells form vessel-like networks independent of endothelial cells—an emerging hallmark of malignancy associated with poor prognosis. This comprehensive laboratory guide provides step-by-step experimental protocols designed to investigate the molecular mechanisms, biomarkers, and therapeutic targets associated with VM.

Edited and authored by leading experts in oncology and molecular pathology, this volume brings together highly reproducible methodologies that span in vitro modeling, 3D culture systems, gene editing techniques, immunohistochemical analysis, and in vivo tumor models.

Key Features:

• Part of the trusted Methods in Molecular Biology series (Volume 2514, Springer)

• Standardized laboratory protocols for studying vasculogenic mimicry

• Detailed methods for cell line establishment, ECM modeling, and gene expression profiling

• Techniques for CRISPR/Cas9 gene editing, immunofluorescence, and 3D tube formation assays

• Explores the role of tumor microenvironment, VE-cadherin, and hypoxia-related signaling pathways

Each chapter includes a concise introduction to the technique, a full list of materials, and step-by-step instructions, along with expert tips and troubleshooting guidance to support reproducibility and experimental success.