Yeast Functional Genomics: Methods and Protocols (Methods in Molecular Biology 2477) 2nd Edition

Yeast Functional Genomics: Methods and Protocols (2nd Edition) from the prestigious Methods in Molecular Biology series (Book 2477) provides a comprehensive, up-to-date resource for researchers exploring the powerful world of Saccharomyces cerevisiae as a model for functional genomics. Now available for order through Books Hub PK, this essential lab manual equips molecular biologists and geneticists with the methodologies needed to decode gene functions at a systems level.

This expanded second edition reflects the rapid advancements in yeast genomics, encompassing a wide range of cutting-edge experimental techniques—from CRISPR-based genome editing to transcriptomics, proteomics, and metabolic pathway analyses. With easy-to-follow protocols, helpful notes, and troubleshooting tips, this volume is indispensable for both newcomers and experienced professionals in the field.

🔬 Key Topics Include:

• High-throughput gene deletion and mutant screening

• CRISPR-Cas9 and RNA interference applications in yeast

• Functional mapping of gene interactions and networks

• Yeast as a model for studying human disease genes

• Omics approaches including transcriptomics and proteomics

• Bioinformatics tools for yeast data analysis

• Real-time phenotypic assays and metabolic profiling