Dengue Virus: Methods and Protocols (Methods in Molecular Biology 2409)
Advance your research on mosquito-borne viral diseases with “Dengue Virus: Methods and Protocols” (Methods in Molecular Biology, Vol. 2409)—a meticulously curated laboratory manual offering the latest techniques, protocols, and tools for studying dengue virus (DENV). Now available at Books Hub PK, this volume is an essential resource for virologists, immunologists, molecular biologists, and public health researchers addressing one of the world’s most urgent infectious disease threats.
Dengue virus remains a major global health concern, especially in tropical and subtropical regions, including South Asia, where it causes frequent outbreaks. This book brings together cutting-edge experimental protocols developed by leading researchers in the field, enabling precise and reproducible studies on dengue virus biology, transmission, pathogenesis, diagnostics, and vaccine development.
Key Features:
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Covers viral genome manipulation, reverse genetics, virus production, and titration techniques
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Detailed protocols for infection models, including in vitro cell culture systems and in vivo mouse models
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Methods for immune response analysis, including ELISA, neutralization assays, flow cytometry, and cytokine profiling
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Tools for molecular diagnostics, including RT-PCR, qPCR, and sequencing
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Strategies for antiviral screening, vaccine research, and vector-host interaction studies
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Each protocol includes step-by-step guidance with materials lists, troubleshooting tips, and expert notes
This volume follows the standard Methods in Molecular Biology format, making it easy to integrate into lab workflows. Each chapter provides a scientific rationale and practical execution steps that are ideal for both early-career and experienced researchers.
