Directed Evolution: Methods and Protocols (Methods in Molecular Biology Book 2461)
Unlock the power of protein engineering with the latest edition of Directed Evolution: Methods and Protocols (Methods in Molecular Biology Book 2461), a must-have guide for scientists and students working in biotechnology, biochemistry, synthetic biology, and pharmaceutical research. Now available for immediate order at Books Hub PK, this book is your gateway to mastering one of the most transformative technologies in modern biology.
Part of the globally respected Methods in Molecular Biology series, this volume offers cutting-edge, peer-reviewed protocols for the design, mutagenesis, selection, and screening of proteins through directed evolution. Whether you’re new to the field or optimizing advanced workflows, these protocols ensure precision, reproducibility, and efficiency.
🔬 Key Features:
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Comprehensive methods for library generation, including error-prone PCR, DNA shuffling, and site-saturation mutagenesis
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Strategies for in vitro and in vivo selection and screening
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Protocols tailored for enzyme optimization, stability enhancement, substrate specificity, and more
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Guidance on using microfluidics, high-throughput screening, and next-gen sequencing (NGS)
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Troubleshooting tips and notes to ensure successful implementation and reproducibility