Effector-Triggered Immunity: Methods and Protocols (Methods in Molecular Biology Book 2523)
Enhance your understanding and experimentation in plant-pathogen interactions with Effector-Triggered Immunity: Methods and Protocols (Methods in Molecular Biology, Vol. 2523). This essential laboratory manual presents a comprehensive collection of validated protocols focused on effector-triggered immunity (ETI)—a critical component of plant immune responses.
Published by Springer under the acclaimed Methods in Molecular Biology series, this volume offers practical guidance for researchers studying host-pathogen dynamics, resistance gene signaling, effector protein function, and downstream plant defense responses. With contributions from international experts, this book equips you with the hands-on tools required to explore one of the most advanced areas of plant molecular biology and immunity.
Each chapter features detailed step-by-step protocols, including required materials, reagent preparation, methodological background, and troubleshooting advice—designed to ensure reproducibility and reliability in a variety of experimental settings.
Key Features:
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Protocols covering cloning and delivery of pathogen effectors,
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Techniques for monitoring immune responses and defense signaling pathways
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Advanced assays for protein-protein interactions, gene expression, and cell death markers
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Methods for studying plant resistance (R) genes and pathogen virulence factors
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Includes biochemical, genetic, and imaging-based techniques
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Covers both model species (e.g., Arabidopsis) and crop plants
This book serves as a must-have guide for academic researchers and applied scientists working on plant immunity, molecular plant pathology, genomics, and agriculture biotechnology.
