Hematopoietic Stem Cells: Methods and Protocols (Methods in Molecular Biology Book 2567)
Hematopoietic Stem Cells: Methods and Protocols (Methods in Molecular Biology Book 2567) is a definitive laboratory guide for scientists, clinicians, and biotechnology professionals engaged in stem cell research, regenerative medicine, and hematology. This volume presents step-by-step experimental protocols for the isolation, characterization, culture, and therapeutic application of hematopoietic stem cells (HSCs), which are critical for the formation and renewal of blood and immune cells.
As part of the prestigious Methods in Molecular Biology series, this book combines expert-authored techniques with detailed instructions, reagent lists, and troubleshooting tips—ensuring accurate and reproducible results in both research and clinical settings. Covering cutting-edge methodologies, it includes protocols for flow cytometry analysis, genetic manipulation, transplantation assays, and ex vivo expansion of HSCs.
Key Features:
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Comprehensive coverage of hematopoietic stem cell isolation and purification.
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Advanced cell culture and expansion techniques for clinical and experimental use.
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Flow cytometry, molecular profiling, and genetic modification protocols.
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Methods for in vivo transplantation and engraftment studies.
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Expert advice on avoiding common experimental pitfalls.
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Fully indexed and cross-referenced for quick laboratory access.
