Membrane Trafficking: Methods and Protocols (Methods in Molecular Biology Book 2473)
Membrane Trafficking: Methods and Protocols is a comprehensive laboratory guide in the respected Methods in Molecular Biology series, offering a complete set of experimental techniques for studying the processes that control the movement of proteins, lipids, and other molecules within cells. Edited by leading experts in the field, this volume provides both foundational methods and advanced protocols to investigate endocytosis, exocytosis, vesicle transport, and organelle dynamics.
This book addresses the central role of membrane trafficking in maintaining cellular organization, signaling, and homeostasis, and how its disruption contributes to diseases such as cancer, neurodegeneration, and immune disorders. Each chapter follows the trusted Methods in Molecular Biology format, featuring an introduction to the topic, a detailed materials list, step-by-step experimental instructions, and expert troubleshooting tips to ensure reproducibility and accuracy.
Key Features of “Membrane Trafficking: Methods and Protocols”:
-
Comprehensive Protocol Collection: Techniques for studying vesicle formation, cargo sorting, membrane fusion, and recycling pathways.
-
Advanced Imaging Methods: Protocols for live-cell imaging, fluorescence microscopy, and super-resolution techniques.
-
Biochemical & Molecular Approaches: Assays for protein-protein and protein-lipid interactions involved in trafficking.
-
Model Systems: Methods applicable to mammalian cells, yeast, and other model organisms.
-
Disease Relevance: Experimental strategies for analyzing trafficking defects in human diseases.
-
Expert Notes: Practical advice to optimize performance and troubleshoot common laboratory challenges.
This resource is ideal for cell biologists, molecular biologists, biochemists, neuroscientists, immunologists, and postgraduate researchers who need reliable, reproducible protocols for studying intracellular transport systems. It serves as both a practical manual for laboratory work and a reference text for designing experiments in membrane biology.